Figure 3.

Percentages of S. aureus PECHA 10 and P. aeruginosa PECHA 4 viability in dual species in forming (A) and mature (B) LCWBs in presence of GO and AMK. For each detection, representative Live/Dead images of in forming and mature LCWBs in presence of GO and AMK compared with the controls, are shown. Original magnification, 1000 × . In Live/Dead images, sessile population in biofilms with a damaged membrane (dead cells) is stained in red, whereas viable cells are green stained. The images observed at fluorescent Leica 4000 DM microscopy are recorded at an excitation/emission wavelengths of 485/498 nm for SYTO 9 and of 535/617 nm for Propidium iodide.