Fig. 7. Altered expression of the miRNA profile in human swMBCs.

a–c Global transcriptional differences of miRNAs in CD27⁺IgG⁺ vs. CD27^–IgD⁺ B cells (a), CD27⁺IgA⁺ vs. CD27^–IgD⁺ B cells (b), and CD27⁺IgG⁺ vs. CD27⁺IgA⁺ B cells (c) as depicted by volcano plot. All miRNAs annotated in human GENCODE v24 GRCh38 are shown, with each circle representing 1 miRNA. −Log₁₀-transformed p is shown on the y axis (p < 0.05 indicated above dashed line). DE miRNAs at p < 0.05 are highlighted in red (upregulated) or blue (downregulated). DE miRNAs at p < 0.05 (with Δ abundance > 1000) shared by CD27⁺IgG⁺ and CD27⁺IgA⁺ are annotated. d Normalized (log₂RPM) expression of the 19 DE miRNAs at p < 0.05 in swMBCs as compared to NBCs depicted by histogram for each B cell subset. Data are mean ± SEM of all three subjects. e Transcriptome clustering of the sorted subsets performed using the 19 DE miRNAs depicted by the PCA plot. Prediction ellipses define 95% confidence intervals. Each symbol represents an individually sorted subset (n = 3). f Relative expression profiles of swMBC core transcriptional signature miRNAs at p < 0.05 compared by heatmap, depicting relative transcriptional changes across CD27^–IgD⁺, CD27⁺IgD⁺, CD27⁺IgG⁺ and CD27⁺IgA⁺ B cell subsets in each subject (order; B, C, G). Data in a–f depict DE miRNAs as determined by edgeR. B cell subsets; CD27^–IgD⁺ (gray), CD27⁺IgD⁺ (lavender), CD27⁺IgG⁺ (purple), and CD27⁺IgA⁺ (dark purple).