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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Cell Chem Biol. 2020 Jul 7;27(8):891–903. doi: 10.1016/j.chembiol.2020.06.010

Figure 4:

Figure 4:

A) Tethered fluorophore-quencher complexes utilize fluorophore-quencher pairs that primarily undergo contact quenching. This enables an aptamer binding event to reduce quenching and turn on fluorescence. B) Fluorophore-quencher pairs are constructed with modern fluorophores linked (usually via PEG) to a quenching moiety.