Table 1.
Plasmodium falciparum multiplication rates in Ghanaian clinical isolates tested in exponential growth assays at different times over five months of continuous culture.
| Patient isolate | Age (yrs) | Parasites µl−1 blood | Hb level g dl−1 | Multiplication rate (95% CI) per 48 h after: | ||
|---|---|---|---|---|---|---|
| Day 25 | Day 77 | Day 153 | ||||
| 271 | 6 | 89,625 | 11.6 | 2.6 (2.1–3.2) | 6.6 (4.8–9.1) | 7.6 (5.5–10.6) |
| 272 | 2 | 47,987 | 9.0 | 4.9 (3.8–6.5) | 4.2 (3.3–5.5) | 5.2 (4.6–6.1) |
| 273 | 3 | 38,056 | 12.0 | 3.8 (2.5–5.6) | 3.3 (2.6–4.2) | 5.9 (4.7–7.2) |
| 274 | 8 | 25,499 | 11.1 | 4.4 (3.3–5.7) | 6.0 (4.4–8.3) | 5.7 (3.5–9.1) |
| 275 | 2 | 20,210 | 9.5 | 3.6 (2.9–4.6) | 3.6 (2.9–4.4) | 4.5 (3.7–5.6) |
| 276 | 7 | 5,985 | 8.8 | 5.6 (5.2–5.9) | 4.4 (3.6–5.3) | 5.2 (3.8–7.2) |
| 277 | 2 | 20,493 | 9.9 | 2.0 (1.7–2.4) | 4.4 (3.3–5.8) | —b |
| 278 | 3 | 92,225 | 8.4 | —a | 6.0 (4.8–7.9) | 10.5 (7.9–13.9) |
| 279 | 8 | 37,410 | 11.5 | —a | 2.8 (2.2–3.5) | —a |
| 280 | 9 | 64,896 | 12.5 | 5.6 (3.5–9.1) | 4.6 (3.6–5.6) | 7.5 (6.2–9.0) |
| 281 | 6 | 2,640 | 11.3 | —a | 5.8 (4.8–7.2) | —c |
| 282 | 5 | 20,776 | 9.5 | 3.1 (2.5–3.8) | 2.8 (2.2–3.5) | 4.7 (3.6–6.2) |
| 284 | 4 | 36,936 | 10.6 | 2.5 (2.3–2.7) | 3.8 (3.2–4.6) | 6.0 (5.1–7.1) |
| 285 | 9 | 11,700 | 13.3 | 3.2 (2.8–3.6) | 3.6 (3.0–4.4) | 5.6 (4.1–7.7) |
| 286 | 7 | 59,304 | 12.0 | 3.6 (3.3–4.0) | 6.9 (4.0–10.0) | 8.7 (6.7–11.3) |
| 287 | 5 | 74,784 | 12.5 | —a | 6.3 (4.8–7.9) | 4.0 (3.4–4.8) |
| 288 | 3 | 44,714 | 10.3 | —a | 4.2 (2.5–6.3) | 6.5 (5.1–8.3) |
| 289 | 9 | 42,512 | 12.4 | 3.8 (2.8–5.1) | 5.5 (4.2–7.6) | —c |
| 290 | 4 | 14,364 | 11.3 | —a | —a | 6.5 (5.4–7.8) |
| 291 | 10 | 15,745 | 9.3 | 2.4 (2.2–2.7) | 6.3 (4.4–9.6) | 4.4 (3.5–5.4) |
| 292 | 3 | 95,370 | 7.1 | 6.9 (5.5–8.7) | 5.5 (3.6–8.3) | 7.3 (5.4–9.9) |
| 293 | 13 | 38,931 | 13.3 | 6.0 (4.4–8.1) | 7.2 (5.0–11.0) | 9.9 (6.9–14.4) |
| 294 | 6 | 61,054 | 11.0 | 5.9 (4.3–8.2) | 6.9 (5.8–8.3) | 6.7 (5.4–8.3) |
| 296 | 2 | 108,931 | 8.4 | 8.0 (6.4–10.0) | 7.2(4.0–10.0) | —c |
All exponential growth assay data for all biological replicates are plotted in full in Supplementary Fig. S1, and numerical data for all measurements are given in Supplementary Data File S1. Confidence intervals on the multiplication rates are derived by logistic regression fitting to exponential growth taking all experimental replicates into account for each isolate (all replicate datapoints shown in Supplementary Fig S1 and Supplementary Data File S1). Missing data for some isolates are indicated by superscripts in the table (aassay did not meet pre-determined replicate requirements for accuracy as outlined in Methods; bculture of one isolate was lost before the final timepoint; ccontamination of three isolates at the final timepoint was detected so data were excluded). Genome sequence accession numbers, and genome-wide complexity measurements of isolates at all timepoints are given in Supplementary Tables S1 and S2.