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. 2020 Oct 28;10:18521. doi: 10.1038/s41598-020-74719-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Human milk microbiota patterns in healthy mothers and mothers suffering lactational mastitis. Constrained correspondence analyses (CCA), which here emphasize variations in microbiota OTU-level patterns, in human milk from healthy mothers and mothers suffering lactational mastitis at two time points, at DNA-level (p = 0.015), and active microbial RNA-level (p = 0.04). The percentage of variation explained by constrained correspondence components is indicated on the axes. t0 = samples during the course of the symptoms; t1 = samples after symptoms cessation. p-values for CCA plots were determined by Adonis, and indicate if health status and/or time can significantly explain data variability. Controls (C): DNA, n = 24; RNA = 23; Mastitis t0: DNA, n = 26; RNA = 26; Mastitis t1: DNA, n = 22; RNA, n = 22. Created in R software v 3.5.1 (2018–07-02) (https://www.R-project.org).