Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 28;10:18433. doi: 10.1038/s41598-020-74128-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

An optimized workflow to efficiently purify and characterize SCs and fibroblasts from adult human peripheral nerve tissue. This protocol uses heterogeneous human cultures containing cells identifiable as SCs and fibroblasts as evidenced by expression of p75^NGFR, a SC (neural crest)-specific receptor. MACS of p75-labelled cells allowed the fast recovery of SCs and fibroblasts retaining their viability and biological activity. The rounds of MACS required to achieve > 95% SC purity was dependent on the degree of fibroblast growth (high or low, as indicated). Immunofluorescence microscopy, flow cytometry and transcriptome (RNA-seq) analysis were used to confirm the identity and purity of the MACS-purified cells. Some key lineage- and stage-specific genes revealed by RNAseq (bottom panel) emphasized the independent origin of human SCs and fibroblasts, their relative immaturity, and the heterogeneity of the fibroblast populations. The genes highlighted in color were confirmed by immunological methods.