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. 2020 Oct 28;11(10):929. doi: 10.1038/s41419-020-03135-z

Fig. 2. MiR-182-5p and miR-378-3p promotes ferroptosis in the renal epithelial cells.

Fig. 2

HK-2 and TCMK-1 cells were treated with miR-182-5p mimics (10 pmol) or inhibitor (40 pmol), or miR-378a-3p mimics (10 pmol) or inhibitor (40 pmol) for 36 h, then the cells were subjected to various detection. A The efficacy of the miRNAs mimics and inhibitors in TCMK-1 and HK-2 cells was verified by qRT-PCR. B Cell viability of TCMK-1 and HK-2 cells were reduced by miR-182-5p and miR-378a-3p mimics, and enhanced by miR-182-5p and miR-378a-3p inhibitors. C Iron level and D lip ROS level of TCMK-1 and HK-2 cells were elevated by miR-182-5p and miR-378a-3p mimics, and decreased by miR-182-5p and miR-378a-3p inhibitors. E miR-182-5p and miR-378a-3p mimics induced increasing of cell death of TCMK-1 and HK-2 cells, while miR-182-5p and miR-378a-3p inhibitors decreased the cell death, as indicated by TUNEL staining (green), Bar = 100 μm. n = 6. Data are presented as the mean ± s.e.m. of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. indicated group.