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. 2020 Oct 28;11(10):929. doi: 10.1038/s41419-020-03135-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Rats renal artery was clamped with non-traumatic clamps for 45 min, followed by restoring of the renal blood flow. Control rats underwent sham surgery (sham group) without ischemia-reperfusion injury (IRI). Blood samples and renal tissues were collected 48 h after the injury. A Serum creatinine and B blood urea nitrogen levels were significantly higher in the I/R group than in the sham group. C Kidney tissue sections were subjected to histological examination by hematoxylin and eosin staining (H&E) and TUNEL assay to evaluate renal tubule injury. D Increased iron level and E ROS level in I/R renal tissues indicates the increase of ferroptosis. F Western blot was used to detect the indicated protein expression. The protein intensity was analyzed by using ImageJ. n = 6. Data are presented as the mean ± s.e.m. of three independent experiments. ***P < 0.001 vs. the sham group.