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. 2020 Oct 28;11(10):929. doi: 10.1038/s41419-020-03135-z

Fig. 5. MiR-182-5p targets GPX4 and miR-378a-3p targets SLC7A11, respectively, in the renal epithelial cells.

Fig. 5

A The predicted binding site between 3′UTR of GPX4 mRNA and miR-182-5p, and the predicted binding site between 3′UTR of SLC7A11 mRNA and miR-378a-3p. B HK-2 cells were co‑transfected with luciferase constructs containing the GPX4 WT or MUT 3′‑UTRs and miR‑182-5p mimics or mimics NC, or co‑transfected with luciferase constructs containing the SLC7A11 WT or MUT 3′‑UTRs and miR‑378a-3p mimics or mimics NC. Luciferase activity was measured. C RIP assay followed by qRT-PCR to assay miR-182-5p and miR-378a-3p endogenously associated with GPX4 and SLC7A11, respectively. D miR-182-5p and miR-378a-3p scarcely influence the GPX4 and SLC7A11 mRNA levels. E The GPX4 and SLC7A11 expression levels were downregulated after treated with miR-182-5p and miR-378a-3p mimics and upregulated with the treatment with miRNAs inhibitors in HK-2 and TCMK-1 cells. The protein intensity was analyzed by using ImageJ. n = 6. Data are presented as the mean ± s.e.m. of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. indicated group.