Figure 4.

Expression of transcripts encoding TCF4-C isoform are downregulated, whereas transcripts encoding other TCF4 isoforms are upregulated in the cornea of FECD patients. Two independent FECD RNA-seq datasets—by Nikitina et al.²⁷ (named 2019 in the figure) and Chu et al.²⁸ (named 2020 in the figure)—were used to analyze the expression levels of TCF4 exons in corneal endothelium. The expression levels of (A) TCF4 isoforms and (B) internal exons were quantified using the number of reads (A) from transcripts encoding for the respective isoform (shown above the graphs) and (B) crossing the indicated splice junction (shown above the graphs). Data was normalized with the total number of spliced reads and multiplied by million. For more details, see legend of Fig. 3.