Table 1.
Main characteristics of HIV-1 infected population from Kinshasa (DRC) with collected DBS in 2016–2018.
| Children/adolescents (%) | Adults (%) | Total (%) | |
|---|---|---|---|
| Subjects with pol sequence | 55 (33.3) | 110 (66.7) | 165 (100) |
| Female | 28 (50.9) | 64 (58.2) | 92 (55.8) |
| Age at DBS collection (years), median [IQR] | 14.4 [11.4–16.8] | 43 [34–53.5] | 32.4 [16.2–48.5] |
| ART exposure | |||
| ARV naive | 0 | 11 (10) | 11 (6.7) |
| ART | 55 (100) | 69 (62.7) | 124 (75.2) |
| Unknown | 0 | 30 (27.3) | 30 (18.2) |
| HIV-1 viraemia>1000 HIV-1-RNA copies at sampling (data known in 55 children and in 103 adults) | |||
| In 1 DBS dot [range] |
19/55 (34.5) [1260–16,700] |
51/103 (49.5) [1200–132,000] |
70 (42.4) [1200–132,000] |
| In 1 ml plasma* [range] |
49/55 (89.1) [1351–469,320] |
86/103 (83.5) [1008–3,901,478] |
135 (81.8) [1008–3,901,478] |
| Available pol HIV-1 sequences | |||
| PR | 36 (65.5) | 79 (71.8) | 115 (69.7) |
| RT | 49 (89.1) | 90 (81.8) | 139 (84.2) |
| IN | 40 (72.7) | 75 (68.2) | 115 (69.7) |
| Combination of sequences by regions | |||
| Only PR | 0 | 1 (0.9) | 1 (0.6) |
| Only RT | 5 (9.1) | 8 (7.3) | 13 (7.9) |
| Only IN | 6 (10.9) | 19 (17.3) | 25 (15.1) |
| PR+RT | 10 (18.2) | 26 (23.6) | 36 (21.8) |
| RT+IN | 8 (14.5) | 4 (3.6) | 12 (7.3) |
| PR+RT+IN | 26 (47.3) | 52 (47.3) | 78 (47.3) |
| HIV-1 variants at pol | |||
| Subtype B | 1 (1.8) | 0 | 1 (0.6) |
| Non-B subtypes | 28 (50.9) | 70 (62.4) | 98 (59.4) |
| CRF | 2 (3.6) | 19 (17) | 21 (12.7) |
| URF | 22 (40) | 17 (14.5) | 39 (23.7) |
| U | 2 (3.6) | 4 (3.6) | 6 (3.6) |
DRC, Democratic Republic of Congo; DBS, dried blood specimens; ART, antiretroviral treatment; ARV, antiretroviral drugs; PR, protease; RT, retrotranscriptase; IN, integrase; pol, HIV-1 pol coding region; CRF, circular recombinant forms; URF, unique recombinants forms; U, unknown variant, not ascribed to pure subtype, CRF or URF.