Table 3.
Evaluation of the dominant-negative potential of somatic TP53 mutations using a yeast-based assay.
| ID | Protein variant | % Net activity (P21, 30 °C) | Classification |
|---|---|---|---|
| 15 patients with Mut/noDel | |||
| MS0273 | p.Arg175His | 61 | D |
| CA0082 | p.Arg213Leu | > 100 | r |
| CF0003 | p.Pro278Arg | 73 | D |
| GM0252 | p.Ile195Thr | > 100 | r |
| MG0248 | p.Arg282Trp | > 100 | r |
| DD0478 | p.Pro191del | > 100 | r |
| GC0448 | p.Arg248Trp | 39 | D |
| PA0254 | p.Ala161Thr | > 100 | r |
| AR0222 | p.His193Arg | 59 | D |
| CG0620 | p.Arg209fs | > 100 | r |
| AA0396 | p.Val157Asp | 61 | D |
| CR0115 | p.Phe113Ser | > 100 | r |
| AG0464 | p.Val173Met | 40 | D |
| SG0168 | p.Ala88fs | > 100 | r |
| DA0455 | p.Arg273His | 32 | D |
Results are shown as percentage of the activity of the co-expression of wild-type (WT) and mutant P53 proteins with respect to the expression of the single WT P53 set as 100%. P53 proteins (WT and mutant) were co-expressed in yLFM-P21-5′ reporter strain and grown at 30 °C. Mutant P53s are classified as dominant (D) or recessive (r), when the net activity is below or above 100%, respectively.