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. 2020 Oct 28;11:5434. doi: 10.1038/s41467-020-19234-6

Fig. 1. IFE differentiation is gradualistic and features numerous transition cells.

Fig. 1

a tSNE plot showing all 16 epidermal subpopulations of the WT P0 mouse back epidermis; IFE cells are in the red-outlined box. HF hair follicle, uHF upper hair follicle, SG sebaceous gland. b Heatmap showing the expression of top 20 marker genes for all 16 epidermal subpopulations. Gene lists in Supplementary Data 1. c Pseudotime analysis of the IFE with cluster identity from a projected on the trajectory. The trajectory goes from basal to transition to differentiated cells without major branches (arrow), consistent with unidirectional IFE differentiation. d Expression of canonical markers for distinct stages of epidermal differentiation. e RNA-FISH for Krt10 and Krt14 in WT P0 mouse epidermis. White arrowheads point to yellow Krt10/Krt14 double-positive cells. E epidermis, D dermis, HF hair follicle. Scale bar = 10 µm, representative image for N = 5. f Expression heatmap and expression pattern of 4,292 pseudotime-dependent genes that cluster into six gene modules. Also shown are the gene ontology and example transcription factors for each gene module. Gene lists in Supplementary Data 1.