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. 2020 Oct 28;6:111. doi: 10.1038/s41420-020-00345-4

Fig. 2. Combination of sempervirine and cisplatin affects TGCT cell growth and induces p53 and MDM2 accumulation.

Fig. 2

A 2102EP(S) (EPS), 2102EP(R) (EPR), and NCCIT sensitivity to different doses of cisplatin in combination with different doses of Sempervirine. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Two-way ANOVA, Dunnett’s multiple comparisons test, n = 3. B Western blot analysis of 2102EP(S) (EPS), 2102EPR (EPR), and NCCIT cell line extracts after 24 h of treatment with sempervirine, cisplatin, or nutlin, for MDM2, p53, and p53-S15P, PARP, cPARP, p21, and RAD51. Tubulin is the reference gene; n = 3.