Table 2.
Antibodies list and dilution.
| Antibody name | Specification | Dilution |
|---|---|---|
| RPA194 | sc-48385 (Santa Cruz Technologies) | 1:1000–1:200 |
| P53 | sc-126 (Santa Cruz Technologies) | 1:1000 |
| MDM2 | OP46 (Sigma-Aldrich) | 1:500 |
| UBF-1 | sc-13125 (Santa Cruz Technologies) | 1:1000 |
| GAPDH | Ab9485 (Abcam) | 1:2000 |
| Tubulin | #T4026 (Sigma-Aldrich) | 1:2000 |
| Nucleolin | sc-8031 (Santa Cruz Technologies) | 1:1000–1:200 |
| Fibrillarin | sc-11336 (Santa Cruz Technologies) | 1:1000 |
| E2F1 | sc-251 (Santa Cruz Technologies) | 1:1000 |
| BrdU | sc-32323 (Santa Cruz Technologies) | 1:1000 |
| PARP | sc-7150 (Santa Cruz Technologies) | 1:1000 |
| P63 | Generous gift of Prof. A. Peschiaroli | 1:1000 |
| P73 | Generous gift of Prof. A. Peschiaroli | 1:1000 |
| Rb | Sc-50 (Santa Cruz Technologies) | 1:1000 |
| RAD51 | sc-9349 (Santa Cruz Technologies) | 1:1000 |
| RNA pol II | sc-899 (Santa Cruz Technologies) | 1:1000 |
| Clathrin | Ab-2731 (Abcam) | 1:1000 |
| γH2AX | #9718 (Cell Signaling Technologies) | 1:500 |
| S6 | #2217 (Cell Signaling Technologies) | 1:1000 |
| Phospho-P53 | #9284 (Cell Signaling Technologies) | 1:1000 |
| P21 | sc-6246 (Santa Cruz Technologies) | 1:1000 |