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. 2020 Oct 28;11:5441. doi: 10.1038/s41467-020-19320-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b Phenotypes of whole plant and grain shape of slg1, WT, and the transgenic T₂ lines of slg1-C, SLG1-RNAi, and SLG1-OE. C, complementation; OE, overexpression; RNAi, RNA interference. Bars = 10 cm (a) and 1 cm (b). c Fine mapping of the SLG1 gene. A single-nucleotide substitution at the splicing acceptor site of the fifth intron of LOC_Os12g39840 in slg1 was indicated. d SLG1 and RCTU1 show high-temperature-inducible expression. Total RNA was extracted from the shoots of 2-leaf-stage seedlings at the indicated time-points. The expression levels were determined by qPCR, and the expression level at time 0 was set to 1. Actin was used as the internal control. Data are means ± SD (n = 3 biological replicates). e–g SLG1 is a positive regulator of thermotolerance. Phenotypes of WT, slg1, SLG1-RNAi, and SLG1-OE plants before and after treatment are shown. Bars = 5 cm. h Dysfunction of RCTU1 shows thermosensitive phenotype similar to the slg1 mutant. Phenotypes of RCTU1-RNAi plants on the background of slg1 and WT are shown before and after treatment. Bar = 5 cm. The source data underlying (d) are provided as a Source data file.