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. 2020 Oct 29;15(10):e0240887. doi: 10.1371/journal.pone.0240887

Fig 3. The asymmetric localization of CAH6-mNG in flagella is lost in the bbs1 mutant.

Fig 3

A) Schematic representation of the CAH6-mNG expression vector. The sequence for mNG was inserted at the 3’ end of the genomic CAH6 coding region. B) Brightfield (BF) images of the flagella (left) and the eyespot (right, indicated with black arrowheads) and the corresponding TIRF images of live cah6 CAH6-mNG and bbs1 cah6 CAH6-mNG cells. The trans-flagella are indicated with red arrowheads and the cis-flagella with yellow arrowheads. Bar = 2μm. C) Histograms of the fluorescence intensity of CAH6-mNG in the trans- and cis-flagella of the cah6 CAH6-mNG rescue strain and the bbs1 cah6 CAH6-mNG strain. For each cell, the fluorescence intensity in its trans-flagellum was set to 100% and the fluorescence intensity of the corresponding cis-flagellum is displayed as % of that of the trans-flagellum. One cell, lacking detectable CAH6-mNG in the cis-flagellum and thus having an outlier trans/cis-ration of 158, was ignored for the statistical analyses. Error bars indicate the standard deviation. n = number of cells analyzed. D) Histogram of the ratio of the fluorescence intensity of CAH6-mNG between the trans- and the cis-flagellum in the cah6 CAH6-mNG and the bbs1 cah6 CAH6-mNG strain; shown is the mean of the ratios determined for the individual trans/cis pairs of individual cells. Error bars indicate the standard deviation and n the number of flagellar pairs analyzed. See S3C Fig for the individual data points.