Table 2. Primer sequences used in this study.
| Gene | Species | Primer sequence (5'-3') | Size (bp) |
|---|---|---|---|
| ET-1 | Human | F: GAGAATTTACTTCCCACAAAGGC R: TCCATAATGTCTTCAGCCCTG |
125 |
| ETAR | Human | F: TGCCCTCAGTGAACATCTTAAG R: CATCGGTTCTTGTCCATCTCG |
147 |
| ETBR | Human | F: CAAGGACCCATCGAGATCAAG R: AGATATTGGGACCGTTTCGC |
145 |
| Col 1α1 | Human | F: GAAGACATCCCACCAATCACC R: TCTCGTCACAGATCACGTCATC |
136 |
| Col 3α1 | Human | F: CTACTTCTCGCTCTGCTTCATC R: CACAGACACATATTTGGCATGG |
136 |
| IL-10 | Human | F: GACAGACTTGCAAAAGAAGGC R: TCTCGAAGCATGTTAGGCAG |
149 |
| GAPDH | Human | F: GGAGCGAGATCCCTCCAAAAT R: GGCTGTTGTCATACTTCTCATGG |
197 |
| ET-1 | Mouse | F: TTCTTGCCGGTTGGGAATGA R: TTTCTACAGAAACCCCGCCC |
118 |
| ETAR | Mouse | F: GTCTTGAACCTCTGTGCTCTC R: GATCCCGATTCCTTGAACTCG |
78 |
| ETBR | Mouse | F: TGAGTATTGACAGATATCGAGCTG R: AAAACCTATGGCTTCAGGGAC |
137 |
| Col 1α1 | Mouse | F: CGCCATCAAGGTCTACTGC R: ACGGGAATCCATCGGTCA |
152 |
| Col 3α1 | Mouse | F: GCCCACAGCCTTCTACACCT R: GCCAGGGTCACCATTTCTC |
110 |
| IL-10 | Mouse | F: CAACATACTGCTAACCGACTCCT R: TGAGGGTCTTCAGCTTCTCAC |
173 |
| GAPDH | Mouse | F: CCTCGTCCCGTAGACAAAATG R: TGTAGTTGAGGTCAATGAAGGG |
139 |