Table 2. Comparison of Leishmania species identification in Peru targeting kinetoplast and nuclear DNAs.
| Target DNA1) | Identification2): numbers | ||||||
|---|---|---|---|---|---|---|---|
| kDNA | Lb or Lp3): 100 | Lg: 14 | Ll: 16 | Ls: 3 | La: 1 | ||
| nDNA | Lb: 43 | Lp: 44 | Lb/Lp: 4 | Lg: 14 | Ll: 15 | Lb: 3 | La: 1 |
| Lg: 6 | Ll: 1 | Lp/Ll: 2 | Lb: 1 | ||||
| kDNA + nDNA | Lb: 43 | Lp: 44 | Lb/Lp: 4 | Lg: 14 | Ll: 15 | Ls-Lb: 3 | La: 1 |
| Lb,p-Lg: 6 | Lp/Ll: 2 | Ll-Lb: 1 | |||||
| Lb,p-Ll: 1 | |||||||
1)kDNA: kinetoplast DNA, nDNA: nuclear DNA. Parasite species were identified by the sequencing analysis of cyt b gene (kDNA) and by the PCR-RFLP analysis of mpi gene (nDNA) fragments with restriction enzymes HaeIII, VpaK11BI, and BstXI. Mito-nuclear discordance was further determined by the comparative analysis of nDNA (hsp70, and 6pgd) and kDNA (COII-ND1) fragments.
2)Lb: L. (V.) braziliensis, Lp: L. (V.) peruviana, Lg: L. (V.) guyanensis, Ll: L. (V.) lainsoni, Ls: L. (V.) shawi, La: L. (L.) amazonensis, Lb/Lp: a putative hybrid of L. (V.) braziliensis and L. (V.) peruviana, Lp/Ll: a putative hybrid of L. (V.) peruviana and L. (V.) lainsoni, Lb,p-Lg: L. (V.) guyanensis with kDNAs of L. (V.) braziliensis or L. (V.) peruviana, Lb,p-Ll: L. (V.) lainsoni with kDNAs of L. (V.) braziliensis or L. (V.) peruviana, Ll-Lb: L. (V.) braziliensis with L. (V.) lainsoni kDNA, Ls-Lb: L. (V.) braziliensis with L. (V.) shawi kDNA
3)L. (V.) braziliensis and L. (V.) peruviana cannot be differentiated by kDNA sequence analysis.