Fig 5. Acinetobactin biosynthetic mutants are impaired for growth under iron restriction.

Wild-type (WT) A. baumannii and its isogenic acinetobactin (ΔbasG), baumannoferrin (ΔbfnL) and fimsbactins (ΔfbsE) biosynthetic mutants were grown in cTMS media with 20% human serum (A), 180 mg/dL human transferrin (B), no added iron source (C), or 30 μM FeCl₃ (D). Bacterial growth was assessed by determining the optical density at 600 nm (OD₆₀₀), at the time points indicated. Data are representative of three independent experiments, and error bars represent the standard error of the mean. Where error bars are not visible, they are shorter than the height of the symbol. Statistical analysis is given for the endpoint growth, as performed by repeated measures two-way ANOVA, where *p < 0.05 and *** p < 0.001. Additional analyses of growth kinetics for these data can be found in S2 Fig.