Table 1. Strains and plasmids employed in this study.
| Strain | Description | Reference |
|---|---|---|
| A. baumannii ATCC 17978 | WT A. baumannii fatal meningitis isolate from 1951 | American Type Culture Collection (ATCC) strain 5377 [54] |
| WT/pWH1266 | WT A. baumannii ATCC 17978 with empty pWH1266 plasmid | This study |
| A. baumannii AB5075-UW | Multidrug-resistant WT A. baumannii AB5075 isolate from a wound infection | [92,93] |
| ΔbasG | Acinetobactin biosynthetic mutant where basG (A1S_2379) was replaced by a kanamycin resistance determinant, and then the cassette was excised, to leave a markerless mutant | This study |
| basG102::Tn26 | Transposon mutant of basG in A. baumannii AB5075-UW from three-allele mutant library; identifier tnab1_kr121128p06q102 | [92] |
| basG142::Tn26 | Transposon mutant of basG in A. baumannii AB5075-UW from three-allele mutant library; identifier tnab1_kr130909p02q142 |
[92] |
| ΔbasG/pWH1266::basG | basG mutant complemented with pWH1266 expressing basG from its native promoter | This study |
| ΔbfnL | Baumannoferrin biosynthetic mutant where bfnL (A1S_1657) was replaced by a kanamycin resistance determinant, and then the cassette was excised, to leave a markerless mutant | This study |
| ΔfbsE | Fimsbactins biosynthetic mutant where fbsE (A1S_2578) was replaced by a kanamycin resistance determinant; KanR | This study |
| ΔbasG bfnL | Acinetobactin and baumannoferrin biosynthetic mutant. Proficient in fimsbactins biosynthesis; KanR | This study |
| ΔbasG fbsE | Acinetobactin and fimsbactins biosynthetic mutant. Proficient in baumannoferrin biosynthesis; KanR | This study |
| ΔbfnL fbsE | Baumannoferrin and fimsbactins biosynthetic mutant. Proficient in acinetobactin biosynthesis; KanR | This study |
| ΔbasG bfnL fbsE | Complete siderophore biosynthetic mutant with disruptions in acinetobactin, baumannoferrin, and fimsbactins production; KanR | This study |
| Δzur | Zur mutant; KanR | [58] |
| Plasmid | ||
| pKD4 | Template for amplification of the FRT-flanked kanamycin resistance determinant; KanR, AmpR | [94] |
| pAT02 | A. baumannii recombinase expressing plasmid; CarbR/AmpR | [61] |
| pAT03 | Expresses an IPTG-inducible flipase to remove the kanamycin resistance determinant; CarbR/AmpR | [61] |
| pWH1266 | A. baumannii/E.coli shuttle and complementation vector; CarbR/AmpR | [95] |
| pWH1266::basG | pWH1266 expressing basG from its native promoter; CarbR/AmpR | This study |