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. 2020 Oct 22;46(6):2057–2068. doi: 10.3892/ijmm.2020.4765

Figure 1.

Figure 1

Effects of honokiol on toxicity and proliferation of fibroblasts. (A) Chemical structure of honokiol. (B) Fibroblasts were incubated with various concentrations of honokiol for 72 h. (C) Fibroblasts were treated with 10 ng/ml TGF-β1, alone or with the indicated concentrations of honokiol for 72 h. (D) Effects of honokiol on cyclin D1, cyclin E and cyclin B1 induced by TGF-β1 were examined by western blot analysis. (E-G) Bar diagram of the cyclin D1, cyclin E and cyclin B1 expression. (H) The active DNA synthesis of fibroblasts was detected by EdU incorporation assay (×100 magnification). (I) Ratios of EdU-positive cells. Data are presented as the means ± SEM, **P<0.01, vs. the control group. #P<0.05, ##P<0.01 compared with the TGF-β1-stimulated group; n=3.