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. 2020 Oct 22;46(6):2057–2068. doi: 10.3892/ijmm.2020.4765

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of honokiol on TGF-β1-induced ECM production by fibroblasts. (A and B) The cellular levels expression of type I collagen and fibronectin were measured by real-time PCR. The expression levels of these genes were normalized to the expression of GAPDH. (C) Fibroblasts treated with TGF-β1, alone or with the indicated concentrations of honokiol, then, harvested for the measurement of type I collagen and fibronectin by western blot. (D and E) Bar diagram of type I collagen and fibronectin expression. Data are presented as the means ± SEM, ^**P<0.01, vs. the control group. ^#P<0.05, ^##P<0.01 compared with the TGF-β1-stimulated group; n=3.