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. 2020 Oct 7;46(6):1973–1982. doi: 10.3892/ijmm.2020.4747

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Copyright: © Feng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Silencing of circCCDC66 suppresses hypoxia-induced CRC cell phenotype. (A) The efficiency of circCCDC66 knockdown in hypoxia-exposed HCT116 and SW620 cells was verified by RT-qPCR analysis. ^*P<0.05 vs. the sh-NC group. (B) CCK-8 assay was conducted to examine cell viability in HCT116 and SW620 cells transfected with sh-NC or sh-circCCDC66 after exposure to hypoxia condition. ^*P<0.05 vs. the sh-NC group. (C and D) The impacts of sh-circCCDC66 on cell invasion and migration of HCT116 and SW620 cells treated with hypoxia were estimated by Transwell assay and wound healing assay. (E) Flow cytometric analysis was used to detect the apoptosis of hypoxia-exposed HCT116 and SW620 cells following circCCDC66 downregulation. ^*P<0.05 vs. the sh-NC group. (F) The protein levels of Bax and Bcl-2 in hypoxia-exposed CRC cells were detected by western blot analysis. CRC, colorectal cancer.