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. 2020 Sep 24;46(6):2115–2125. doi: 10.3892/ijmm.2020.4736

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Copyright: © Zhu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Characterization of OCEXs. (A) The sizes of the WSU-HN4- and SCC-9-derived exosomes were determined by NTA. (B) Representative images of exosomes derived from WSU-HN4 and SCC-9 cells, as detected by TEM. Scale bars, 50 nm. (C) Expression of the exosomal markers, Alix, CD63, CD9 and Rab5, in WSU-HN4- and SCC-9-derived exosomes was determined by western blot analysis. (D) Co-expressed proteins in WSU-HN4- and SCC-9-derived exosomes were detected by mass spectrometry. (E) Overlap of OCEX proteins and the top 100 frequently identified exosomal protein markers from the ExoCarta database. OCEXs, oral cancer-derived exosomes; NTA, nanoparticle tracking analysis; TEM, transmission electron microscopy; EXO/Exo, exosomes.