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. 2020 Sep 30;46(6):2282–2284. doi: 10.3892/ijmm.2020.4743

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Immunohistochemical staining. Stronger positive expression (brown-yellow color) of COL-II and weaker expression of COL-I was observed in the nHC group compared with that in the HC and pure collagen groups, indicating that the chondrcocyte phenotype and biological functions could be maintained effectively when cells were cultured on nHC film for a long time period. HC, hydroxyapatite/collagen; nHC, nano-HC; calcein-AM/PI, acetoxymethyl/propidium iodide; TGF-β, transforming growth factor-β; COL-I, collagen type I; COL-II, collagen type II.