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. 2020 Oct 9;46(6):2078–2088. doi: 10.3892/ijmm.2020.4749

Figure 2.

Figure 2

miR-202 overexpression inhibits the invasion and migration of ESCs. ESCs were transfected with miR-202 mimics or mimics NC for 24 h, following which cells were harvested for subsequent experiments. (A) The verification of primary cultured ESCs by using vimentin and cytokeratin 7. Magnification, ×100. (B) Transfection efficiency of miR-202 mimics was assessed by reverse transcription-quantitative PCR in ESCs. (C) Cell invasion and (D) wound healing assay were performed to measure the invasion and migration of ESCs, respectively. Magnification, ×200. Data represent the mean ± SD from three independent experiments. **P<0.01 vs. mimics NC. (E) The expression of E-cadherin and N-cadherin proteins were measured by western blotting. ESCs, endometrial stromal cells isolated from ectopic endometriosis tissues; miR, microRNA; NC, negative control.