Fig. 2.

Media and passage wise effects on osteogenesis specific gene expression in pMSC. Relative mRNA expression of ALP, COL1A1, SPP1 and Bone gamma carboxyglutamate (gla) protein (BGLAP) in pMSC derived and grown in αMEM (A), aDMEM (D), M199 (M), αMEM/M199 (A/M), aDMEM/M199 (D/M), αMEM/aDMEM (A/D) media at passage 5 (P5), passage 10 (P10) and overall. The GAPDH was used as an endogenous control and the fold changes of gene expression were calculated using the method of Pfaffl (2004) considering αMEM expression as calibrator. Values under each bar are mean±s.e.m. of triplicate samples from three animals. Different letters ‘a to f’ (P5), ‘i to n’ (P10) and ‘u to z’ (overall) indicated on top of each bar represented significant difference at P≤0.05 between media. The ‘*’ on top of each bar represented significant difference at P≤0.05 between the passages.