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. 2020 Oct 19;9:e59022. doi: 10.7554/eLife.59022

Figure 5. NLRC4 expression in IECs is sufficient to prevent shigellosis.

Figure 5.

(A) Schematic of the B6 Rosa26 locus containing the iNlrc4 cassette, as described previously (Rauch et al., 2017). (B–F) Vil1-cre positive (+) or negative Nlrc4–/– iNlrc4 littermates, or iNlrc4+/– mice were orally infected with 5x107 CFU of WT Shigella 24 hr after oral streptomycin treatment. Endpoint harvests were done 48 hr post-infection (p.i.). (B) Representative images of the cecum and colon dissected from iNlrc4 Nlrc4–/–Vil1-cre positive or negative mice. (C) Quantification of cecum length reduction normalized to the weight of the animal prior to infection; cecum length (cm) / mouse weight (g). (D) MPO levels measured by ELISA of feces collected -1 through 2 days p.i. (E) Representative images of H&E stained cecum and colon tissue from infected mice. Scale bar, 200 μm. (F) Blinded quantification of histology score (cumulative) for cecum and colon tissue. Data are representative of two independent experiments. Mean ± SD is shown in (C, D, F), Mann-Whitney test, *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant (p > 0.05). (C,D,F) Each symbol represents one mouse.