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. Author manuscript; available in PMC: 2020 Oct 29.
Published in final edited form as: Lab Chip. 2020 Aug 3;20(16):2872–2888. doi: 10.1039/d0lc00207k

Figure 1: Thermal-crosslinking of PEM+DOTAP has similar cell retention to formaldehyde crosslinking DOTAP to PEM.

Figure 1:

A) Diagram of improved tethering surface with1 bilayer and the addition of thermal crosslinking. B-D. Percent of tethered cell retention after sequentially washing B) MDA-MB-436, C) MDA-MB-231TD and D) MCF7 cells on microfluidic slides coated with (PMA/PAAm)4+DOTAP with formaldehyde (CH2O)-crosslink or thermal-crosslinked (PMA/PAAm)1+DOTAP (heat). Data are shown as mean ± SEM, n=3; NS, P > 0.05 vs. (PMA/PAAm)1 + heat (two-way ANOVA with Bonferroni posttest). E) Representative images of tethered MDA-MB-436 cells stained with NucBlue Live ReadyProbes Reagent on (PMA/PAAm)4+DOTAP with formaldehyde-crosslink and (PMA/PAAm)1+DOTAP with thermal-crosslink microfluidic slides after washing. Images were taken at 4X magnification in the DAPI channel on the Nikon Eclipse Ti2-E inverted microscope. Scale bar = 500μm.