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. Author manuscript; available in PMC: 2020 Oct 29.
Published in final edited form as: Lab Chip. 2020 Aug 3;20(16):2872–2888. doi: 10.1039/d0lc00207k

Figure 6: Efficient tethering of CTCs recovered from blood samples and device shelf-life.

Figure 6:

A) Diagram of the process of CTC isolation in Vortex’s VTX-1 with a direct output onto TetherChips, which allows for downstream analysis of suspended cells. B) Representative image of MDA-MB-231TD cells that were spiked into whole blood, isolated in the VTX-1, tethered onto a TetherChip surface (thermal-crosslinked (PMA/PAAm)1+DOTAP) and then fixed and stained with Hoechst 33258 (1:5000) and WGA (1:100). Images were taken at 60X magnification using an Olympus IX81 microscope with a Fluoview FV1000 confocal laser scanning system. Scale bar = 20μm. C) Percent of cell retention after tethering, fixing and staining of MDA-MB-231TD cells that were spiked into whole blood, isolated in the VTX-1, and delivered to a TetherChip surface. Data are shown as mean ± SEM. D) Percent of tethered cell retention after sequentially washing MDA-MB-231TD cells on TetherChips that had been stored in a glass desiccator or on the benchtop for the indicated time points. Data are shown as mean ± SEM, n=1 with triplicates.