FIGURE 3.

E2F7 was overexpressed in pancreatic ductal adenocarcinoma (PDAC) and is a target of miR‐10b. A and B, E2F7 was overexpressed in ASPC‐1, and T3M4 compare to it in HPDE6‐C7 by RT‐PCR and western‐blot. C, PDAC cells were transfected with the mimics of miR‐145. D, With the transfection of miR‐10b mimics, the protein level of E2F7 was decreased. E, The binding sites between miR‐10b and the E2F7 transcript. F, Luciferase activities were measured in ASPC‐1 cells co‐transfected with luciferase reporter containing E2F7 and the mimics of miR‐10b or mutant. G, H, The overexpression of E2F7 contributed to the poor DFS and OS of GC patients. Data are presented as the relative ratio of renilla luciferase activity and firefly luciferase activity. Data are shown as mean ± SD, n = 3. The Student's t test assesses statistical data significance. *P < .05, **P < .01