Table 2.
Results of HPLC method validations for five triterpenes.
| Triterpenes | Linearity equation | r2 | Test range (ng) | LOQ (ng) | LOD (ng) | Intraday precision (RSD, %) | Interday precision (RSD, %) | Recovery (%, n = 6) |
|---|---|---|---|---|---|---|---|---|
| Asiaticoside | y = 5749x + 8480 | 0.9996 | 50–5000 | 1.5 | 0.5 | 1.64 | 1.45 | 99.3 ± 1.01 |
| Asiaticoside B | y = 3609x + 12,781 | 0.9998 | 50–5000 | 2 | 1 | 0.53 | 1.62 | 99.6 ± 1.37 |
| Madecassoside | y = 6278x-3486 | 0.9998 | 50–5000 | 2 | 1 | 1.31 | 1.68 | 98.4 ± 1.25 |
| Madecassic acid | y = 24076x + 13,033 | 0.9996 | 20–2000 | 5 | 2 | 1.09 | 1.17 | 98.7 ± 1.48 |
| Asiatic acid | y = 25974x + 4978 | 0.9996 | 10 -1000 | 8 | 3 | 0.86 | 1.31 | 98.2 ± 1.73 |