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. 2020 Oct 29;10:18674. doi: 10.1038/s41598-020-75675-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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IL-18BP decreased GDF15 and increased citrulline levels in mouse serum after TBI. Mice received single dose of rhIL-18BP (2 mg/kg) or vehicle-control SC injection 48 h post-sham (0 Gy) or 9.4 Gy TBI and sera were collected on day 3, 4 and 7 after TBI. Levels of (a) GDF15 and (b) citrulline in mouse serum were measured with ELISA and Colorimetric kit. Results were obtained from two independent experiments (6 mice/group/experiment, N = 12). Means ± SD. Level of increase *p < 0.05; **p < 0.01; rhIL-18BP-treated vs. vehicle-treated.