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. 2020 Oct 29;10:18677. doi: 10.1038/s41598-020-75807-x

Figure 1.

Figure 1

Immunohistochemically staining of MT1/2 before and after cisplatin treatment of cell lines. MPM cell lines MSTO-211H, NCI-H2052 and NCI-H2452, as well as the lung fibroblast cell line MRC-5 were stained for MT1/2. On the basis of these figures, scoring of MT expression was performed as shown in Table 1. Cisplatin-treated cells were exposed to 10 µM cisplatin for 24 h and then harvested for formalin fixation and FFPE embedding. MRC-5 cells sowed no MT expression, while MSTO-211H showed minimal expression and both cell lines did not show changes in MT expression after cisplatin treatment. Both cell lines NCI-H2052 and NCI-H2452 showed moderate MT-expression when untreated and an increased expression after cisplatin treatment.