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. 2020 Oct 29;10:18677. doi: 10.1038/s41598-020-75807-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Study design of siRNA knockdown-experiments. Cell lines were treated with siRNA for knockdown of MT1A, MT1B and MT2A expression. Cells were treated with cisplatin and, after 48 h of incubation, were analyzed for apoptosis, necrosis and viability. Additionally, FFPE specimens were generated for gene- and protein expression analysis. As we generated one FFPE specimen for each condition in each cell line.