Fig. 5. Zbtb11 depletion results in impaired mitochondrial respiration.

a Changes in mitochondrial membrane potential (MMP) caused by Zbtb11 depletion. Control and Zbtb11 KO cells were stained with TMRE (tetramethylrhodamine, ethyl ester) at the indicated time following KO induction. Mean fluorescence intensity obtained by flow cytometry and their means are shown in samples without (left) and with 4 μM FCCP (right). Individual data points and means are shown for n = 6 (no FCCP) and n = 5 (FCCP-treated) independent experiments, respectively. P values for paired two-tailed t tests are shown above. See also Supplementary Fig. 5b for full FCCP titration curves. b Decreased mitochondrial respiration in Zbtb11 KO cells. Zbtb11^lox/lox Rosa26^ERt2-Cre cells were treated with either EtOH or 4OHT for 48 or 72 h, and oxygen consumption rates (OCR) were measured using the Seahorse platform before and after the sequential addition of the ATP synthase inhibitor oligomycin (O), the mitochondrial uncoupler FCCP, and a mix of rotenone (complex I inhibitor) and antimycin A (complex III inhibitor) (Rot/AA). Upper panels—OCR values adjusted for gDNA content (measured by qPCR and used as a proxy for cell number), showing mean ± SEM of 8 (48 h) and 7 (72 h) biological replicates, and a total of 44–48 measurements (technical × biological). Lower panels—mitochondrial respiration parameters (mean ± SD) in control and Zbtb11 KO cells, calculated from OCR measurements (see Methods). P values (Wilcoxon’s rank-sum test) are indicated above each pair.