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Enhanced VEGF-A binding affinity of engineered aflibercept variants. Bio-layer interferometry-based measurements of the interaction kinetics of soluble Fc-fused aflibercept and Fc-fused aflibercept variants (C5, D4, and F3) with immobilized VEGF-A. All soluble proteins were used at a concentration of 100 nM. Response signals were normalized to their respective maximum values.
