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. 2020 Jun 24;13(5):447–461. doi: 10.1007/s12195-020-00626-z

Figure 4.

Figure 4

MAD1 integration into cancer cells and subcellular trafficking. (a) Confocal micrographs of OVCAR-3 ovarian carcinoma cells treated with 14 μM of fluorescein-labeled MAD1 for 1 h. (b) Magnification of boxed cell in merged image of panel a. Membrane ruffling marked by white arrows. (c) Micrographs of OVCAR-3 cells following a 10 h incubation with MAD1. (d) Magnification of boxed cell in merged image of panel c demonstrating peptide localization to the nuclear envelope (see Supplementary Fig. 9 for 3D z-stacks). Scale bars for panels a-d = 15 μm. (e) SEM image of membrane-templated MAD1 pores following a 1 h treatment of OVCAR-3 cells with MAD1. Peptide-induced surface pores highlighted by red arrows. (f) Magnification of membrane pores formed by MAD1. Inset: Histogram of pore diameter. (g) Magnified SEM micrograph of an OVCAR-3 cell treated for 4 h with MAD1 (full image can be found in Supplementary Fig. 11). Cell membrane (orange) and nucleus (blue) have been false-colored to aid visualization. Scale bar for panels (e)–(g) = 5 μm.