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. 2020 Oct 16;11:577823. doi: 10.3389/fimmu.2020.577823

Figure 5.

Figure 5

TLR4 stimulatory activity of synthetic MPL adjuvant® components. Synthetic versions of several of the major components of MPL adjuvant® were tested for TLR4 stimulatory activity using HEK-Blue reporter cells that expressed either (A) human TLR4 or (B) mouse TLR4. Values shown are averages ± SD from three (A) or two (B) independent experiments normalized by setting 100% = top dose plateau of PHAD in each experiment. Shaded regions indicate 99% confidence intervals within which the true population means should occur 99% of the time. TNFα secretion by (C) human THP-1 and (D) mouse RAW264.7 cells as measured by ELISA 24 hrs after exposure to agonists in triplicate pooled from two or three independent experiments, respectively, with the exception of ML4A, ML4B and ML3 which were tested in triplicate in one experiment with RAW264.7 cells. Overall, ML5A and ML6 are weaker agonists of human TLR4 than of mouse TLR4, relative to the full agonist PHAD, whereas ML4A is an agonist only of mouse TLR4.