. 2020 Oct 16;7:588334. doi: 10.3389/fvets.2020.588334

Table 1.

Activation of apoptotic pathways in vitrified and fresh domestic cat oocytes, assessed as DNA fragmentation (TUNEL-positive oocytes) and caspase activity (3 replicates).

Oocytes	n.	DNA fragmentation % (n.)	Caspase activity Fluorescence intensity mean value ± SD
2hVOs	31	29 (9)^a	321.7 ± 212.3^a
24hVOs	32	59.4 (19)^b	414.6 ± 326.8^a
FOs	31	9.7 (3)^a	199.6 ± 178.3^b
HPOs	33	90.9 (30)^c	420.1 ± 346.1^a

Different superscripts (a, b, c) within the same column indicate significant differences among groups (p < 0.05).

2hVOs, vitrified oocytes incubated in Medium 199 for 2 h after warming; 24hVOs, vitrified oocytes incubated in Medium 199 for 24 h after warming; FOs, fresh oocytes (negative control); HPOs, oocytes treated with hydrogen peroxide to induce apoptosis (positive control).