. 2020 Oct 16;7:588334. doi: 10.3389/fvets.2020.588334

Table 2.

Activation of apoptotic pathways in domestic cat vitrified oocytes (VOs), vitrified-warmed and/or incubated after warming with the addition of the pan-caspase inhibitor Z-VAD-FMK, assessed as DNA fragmentation (TUNEL-positive oocytes) and caspase activity (3 replicates).

Oocytes	n.	DNA fragmentation % (n.)	Caspase activity Fluorescence intensity mean value ± SD
VOs(+/+)	34	8.8 (3)^a	243.7 ± 106.9^a
VOs(+/−)	37	37.8 (14)^b	653.9 ± 591.6^b
VOs(−/−)	36	69.4 (25)^c	434.5 ± 248.3^b

Different superscripts (a, b, c) within the same column indicate significant differences among groups (p < 0.05).

VOs(+/+), oocytes vitrified-warmed and incubated for 24 h with the addition of Z-VAD-FMK; VOs(+/−), oocytes vitrified-warmed with the addition of Z-VAD-FMK and incubated for 24 h in plain Medium 199; VOs(−/−), oocytes vitrified-warmed and incubated for 24 h in Medium 199-based media without the addition of Z-VAD-FMK (control).