Table 3.
Oocytes | n. |
Maturation† % (n.) |
Fertilization‡ % (n.) |
Deg/N.A. % (n.) |
---|---|---|---|---|
VOs(+/+) | 128 | 53.1 (68)a, b | 44.5 (57)a | 35.2 (45)a |
VOs(−/−) | 129 | 47.3 (61)a | 41.1 (53)a | 38.8 (50)a |
FOs | 130 | 65.4 (85)b | 52.3 (68)a | 13.9 (18)b |
Different superscripts (a, b) within the same column indicate significant differences among groups (p < 0.05). Percentages calculated on the total number of oocytes in each group.
VOs(+/+), oocytes vitrified-warmed and in vitro matured with the addition of the pan-caspase inhibitor Z-VAD-FMK; VOs(−/−), oocytes vitrified-warmed and matured without the inhibitor Z-VAD-FMK (control); FOs, fresh control oocytes; Deg, degenerated; N.A., not assessable.
Sum of cleaved embryos, uncleaved fertilized oocytes (i.e., with pronuclei) and unfertilized metaphase II oocytes.
Sum of cleaved embryos and uncleaved fertilized oocytes.