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. 2020 Oct 16;11:574775. doi: 10.3389/fpls.2020.574775

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Copyright © 2020 He, Wu, Liu, Jiang, Yu, Qiu, Wang, Zhang and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enzyme activity analysis of TaUGT6 in DON glucosylation. GST or GST-tagged TaUGT6 proteins were added to the reaction mixture with UDP-glucose as the sugar donor and DON as the substrate and analyzed using HPLC–MS/MS. HPLC–MS/MS analysis of the DON standard substance (A) and DON-3-O-glucoside standard substance (B). HPLC–MS/MS analysis of DON (C) and D3G (D) in the reaction mixture with GST after 3 hours reaction. The reaction mixture with GST-tagged TaUGT6 proteins after 3 hours reaction was analyzed, and DON (E) and D3G (F) were detected.