Skip to main content
. 2020 Jul 10;11(1):77–98. doi: 10.1016/j.jcmgh.2020.07.003

Figure 3.

Figure 3

C57BL/6 and in-house Muc2–/–have different bacteria phyla distribution. (A) Diagrammatic work plan. Briefly, Muc2+/+ and Muc2–/– antibiotic-treated mice received FMT every other day before giving DSS in drinking water. Muc2+/+ received 3.5% DSS for 5 days while Muc2–/– received 1% DSS for 3 days, followed by ad libitum water. Mice were sacrificed on day 14 following DSS treatment. (B) The 16s rRNA sequencing comparison of bacterial phyla abundance in Muc2+/+ and Muc2–/– littermates receiving either homologous or opposite genotype FMT. Analysis shows the results of day 1 post-FMT. (C) Nonmetric multidimensional scaling (NMDS) analysis of samples was performed to plot population distribution between each sample, in which a clear separation between groups is seen. (D) Muc2–/– and Muc2+/+mice were treated for 17 days with an antibiotic cocktail and received FMT with either Muc2–/– or Muc2+/+. Animals were sacrificed 1, 5, and 10 days after FMT. No significant differences in weight change were observed between the 4 groups. (E) Muc2+/+ receiving Muc2–/– FMT showed no significant increase in proinflammatory cytokines compared with control animals receiving their own FMT. Relative expression as compared with its own untreated control animal. n = 3–4, ∗P < .05, ∗∗P < .01. Abx, antibiotics; BW, body weight.