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. 2020 Oct 16;11:507036. doi: 10.3389/fmicb.2020.507036

FIGURE 3.

FIGURE 3

The primary infection of P. brassicae on susceptible and resistant host plants of B. rapa. The primary infection of P. brassicae was investigated in susceptible host plants of ECD5 and resistant host plants of ECD4. (A) FCM images of the zoosporangia and secondary zoospores in the root epidermal cells of susceptible host plants at 7 dpi. Live cell dyes HLG, DAPI and FM4-64 were used to label lipid droplets, nuclei, and plasma membrane of P. brassicae parasites, respectively. The part highlighted with a white box was enlarged for a view in the lower panel. (B) FCM images of the zoosporangia and secondary zoospores in the root epidermal cells of resistant host plants at 7 dpi. Live cell dyes HLG, DAPI, and FM4-64 were used to label lipid droplets, nuclei, and plasma membrane of P. brassicae parasites, respectively. The part highlighted with a white box was enlarged for a view in the lower panel. The part highlighted with a black box in the bright field (lower panel) was further enlarged for a view in C. (C) A free secondary zoospore was profiled. The head and long flagellum of secondary zoospore were indicated with black arrows. Scale bars: 10 μm.