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. 2020 Oct 31;56:58–68. doi: 10.1540/jsmr.56.58

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©2020 The Japan Society of Smooth Muscle Research

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2. — Musculature of the large intestine of BALB/c and W^sh/W^sh mice.

ICC were observed through immunofluorescence, showing TMEM16A immunoreactivity (green) and c-Kit ACK4 immunoreactivity (red) in both BALB/c (A, C, E) and W^sh/W^sh (B, D, F) mice. In the cecum (A, B), ICC-IM and ICC-MY (arrows) in BALB/c mice were observed to have TMEM16A and c-Kit immunoreactivities, and only ICC-MY (arrows) in W^sh/W^sh mice were observed to have TMEM16A immunoreactivity. In the proximal colon (C, D), ICC-SM (double arrowheads) and ICC-MY (arrows) showed TMEM16A immunoreactivity in W^sh/W^sh mice. In the distal colon (E, F), ICC-SM (double arrowheads) exhibited TMEM16A immunoreactivity in W^sh/W^sh mice. c and l represent circular and longitudinal muscle layers, respectively. Asterisks show myenteric layer. Bar: 50 μm.