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. 2020 Oct 31;56:58–68. doi: 10.1540/jsmr.56.58

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©2020 The Japan Society of Smooth Muscle Research

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6. — Developmental changes of intestinal musculature in BALB/c and W^sh/W^sh mice.

In embryos of both 14.5 (E14.5) and 18.5 (E18.5) days, the small intestine was observed using c-Kit ACK4 (green) and SMA (red) antibodies. In the BALB/c intestine (A, C), c-Kit immunopositive cells were clearly observed in the myenteric (arrows) and longitudinal layers in E14.5 (A) and myenteric layer (arrows) in E18.5 (C). In the small intestines of W^sh/W^sh mice (B, D), c-Kit immunoreactivity was not observed in both E14.5 (B) and E18.5 (D). The muscular structures of both mice stained with the SMA antibody appeared to be closely similar. Bar: 50 μm.