Skip to main content
. 2020 Oct 16;11:594496. doi: 10.3389/fphar.2020.594496

Figure 5.

Figure 5

Ursodeoxycholic acid (UDCA) mitigated As(III)-induced cytotoxicity in LO2 hepatocytes. (A) LO2 cells cells were stimulated with 0.05 to 20 ppm As(III) and cell viability was evaluated at different time intervals. (B) The cells were treated with UDCA of escalating concentrations for 24 h, followed by assessment of cell viability. (C) The cells were pre-treated with 0 to 120 μM of UDCA for 24 h, and then incubated with 1 ppm As(III) for another 24 h by using CCK-8 assay. (D) Lactate dehydrogenese (LDH) leakage into the culture medium of LO2 cells with/without 1 ppm As(III) in the presence or absence of treatment pretreatment with 0 to 120 μM UDCA measured by non radiative cytotoxicity assay kit. Data are presented as Mean ± SEM (n = 6). Significant differences are shown as **P < 0.01, compared with the control group. # P < 0.05, ## P < 0.01, compared with the As(III) group.