Figure 6.

Ursodeoxycholic acid (UDCA) attenuated As(III)-induced LO2 hepatocytes death via Nrf2 activation. LO2 cells were transfected with NC siRNA or Nrf2 siRNA for 48 h, and then incubated with UDCA, and then subjected to As(III) stimulation. (A) After drug treatment, the cells were stained for Nrf2 subunit, whereas the cell nuclei were stained with DAPI. The cells were imaged under fluorescence microscopy. Scale bar: 100 μm (magnification 200×). (B) Protein expressions of Nrf2 proteins in nuclear extracts, Lamin B was used as a protein control to normalize volume of protein expression. Nrf2 antioxidant-related proteins, including (C) total Nrf2, (D) HO-1, (E) NQO1, (F) Keap-1 in total lysates were also analyzed by were analyzed by western blotting with specific antibodies. Representative blots were shown. Lamin B and GAPDH was used as loading control. Data are presented as Mean ± SEM (n = 3). (G) Cell viability was evaluated by CCK-8. Data are presented as Mean ± SEM (n = 6). Significant differences are shown as **P < 0.01, compared with the control group. ^# P < 0.05, ^## P < 0.01, compared with the As(III) group. ⁺⁺ P < 0.01, ⁺ P < 0.05, compared with the As(III)+UDCA group *P < 0.05.